Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	336,246	T→C	V82A (GTG→GCG)	yahF →	predicted acyl‑CoA synthetase with NAD(P)‑binding domain and succinyl‑CoA synthetase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	336,246	0	T	C	100.0%	98.6 / NA	54	V82A (GTG→GCG)	yahF	predicted acyl‑CoA synthetase with NAD(P)‑binding domain and succinyl‑CoA synthetase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/54);  total (0/54)

CTGGTGGAGATTGAAGAACTGTTCAACACCAAAGCGC  >  W3110S.gb/336242‑336278     |                                 cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:71428/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:58409/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:58800/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:58878/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:59346/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:5985/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:63622/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:64827/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:65176/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:6579/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:68668/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:69311/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:69919/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:70472/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:57116/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:72293/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:72941/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:74239/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:74608/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:75157/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:80180/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:81121/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:81147/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:85100/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:86219/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:88006/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:9857/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:36774/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:10908/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:133/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:13722/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:15772/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:18288/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:22698/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:24707/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:25144/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:26891/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:31674/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:32966/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:35137/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:35217/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:10735/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:38104/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:3951/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:41993/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:4477/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:46686/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:49538/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:51640/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:52584/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:54103/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:55933/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:56008/37‑1 (MQ=255) cTGGCGGAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:5613/37‑1 (MQ=255)  tggcggAGATTGAAGAACTGTTCAACACcaaagcgc  <  1:68034/36‑1 (MQ=255)     |                                 CTGGTGGAGATTGAAGAACTGTTCAACACCAAAGCGC  >  W3110S.gb/336242‑336278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑