Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,069,469	G→A	I144I (ATC→ATT)	fbaA ←	fructose‑bisphosphate aldolase, class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,069,469	0	G	A	100.0%	50.0 / NA	30	I144I (ATC→ATT)	fbaA	fructose‑bisphosphate aldolase, class II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/0);  total (30/0)

GCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGA  >  W3110S.gb/3069427‑3069483                                           |               gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:1572/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:87666/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:87364/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:85699/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:84714/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:82726/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:79829/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:76863/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:74861/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:71775/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:70030/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:69757/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:69082/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:67360/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:67083/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:64928/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:61862/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:56378/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:55978/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:55543/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:43901/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:40564/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:39438/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:31247/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:2985/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:20847/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:16963/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaaga  >  1:13802/1‑57 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaag   >  1:40446/1‑56 (MQ=255) gCAGATTTCAATGTTCTCTTGCAGAGATTCTTCAGACAGGTCAATCATGTgagaag   >  1:9858/1‑56 (MQ=255)                                           |               GCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGA  >  W3110S.gb/3069427‑3069483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑