Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,079,326	G→A	A322A (GCC→GCT)	tktA ←	transketolase 1, thiamin‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,079,326	0	G	A	100.0%	69.1 / NA	39	A322A (GCC→GCT)	tktA	transketolase 1, thiamin‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/39);  total (0/39)

GCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCGGCTTCCTGCG  >  W3110S.gb/3079276‑3079336                                                   |           gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGTGTAAATTCAGCAGCTTCCTGCg  <  1:61075/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:66405/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:47122/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:53624/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:54918/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:56542/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:56708/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:59105/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:64153/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:50295/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:6830/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:69644/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:75234/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:76425/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:76488/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:799/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:82652/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:87975/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:3866/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:17630/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:18789/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:18922/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:2364/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:25457/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:25823/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:31787/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:34734/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:37035/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:46249/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:42113/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:42289/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:45287/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:4563/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:45722/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:45890/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTTCGCCTTTCATACAGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:53088/61‑1 (MQ=255) gCGTCGAAGTCAGACGGCATTACGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:52583/61‑1 (MQ=255)            aGACGGCATTTCGCGTTTCATACGGCGGGTAAATTGAGCAGCTTCCTGCg  <  1:6987/50‑1 (MQ=255)                     ttCGCCTTTCATACGGCGGGTAAATTCAGCAGCTTCCTGCg  <  1:14870/41‑1 (MQ=255)                                                   |           GCGTCGAAGTCAGACGGCATTTCGCCTTTCATACGGCGGGTAAATTCAGCGGCTTCCTGCG  >  W3110S.gb/3079276‑3079336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑