Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	336,315	G→C	G105A (GGC→GCC)	yahF →	predicted acyl‑CoA synthetase with NAD(P)‑binding domain and succinyl‑CoA synthetase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	336,315	0	G	C	100.0%	106.2 / NA	56	G105A (GGC→GCC)	yahF	predicted acyl‑CoA synthetase with NAD(P)‑binding domain and succinyl‑CoA synthetase domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (56/0);  total (56/0)

CGCACGAGGCGCGTTACGCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGT  >  W3110S.gb/336288‑336343                            |                             cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAg   >  1:51140/1‑55 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAg   >  1:40087/1‑55 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:63041/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:46339/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:477/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:52257/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:54135/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:54302/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:55339/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:56442/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:57341/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:58269/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:58905/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:6236/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:62568/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:461/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:65728/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:66657/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:68187/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:6979/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:71001/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:71067/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:71930/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:71948/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:78781/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:83675/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:83898/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:84441/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:9961/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:28128/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:11053/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:13740/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:14726/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:15812/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:16549/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:1677/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:18069/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:21625/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:22070/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:22545/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:26245/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:2788/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:10074/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:29724/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:33529/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:33719/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:35074/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:36404/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:3688/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:37615/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:38917/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:39185/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:43671/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:43706/1‑56 (MQ=39) cGCACGAGGCGCGTTACGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:44807/1‑56 (MQ=39) cGCACGAGGCGCGTTAAGCCACTATTGCCAGCGCCAAAAAGTATATCCCGGAAAGt  >  1:26763/1‑56 (MQ=38)                            |                             CGCACGAGGCGCGTTACGCCACTATTGGCAGCGCCAAAAAGCATATCCCGGAAAGT  >  W3110S.gb/336288‑336343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑