Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,118,412	G→A	S508S (TCC→TCT)	yghK ←	glycolate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,118,412	0	G	A	100.0%	86.7 / NA	48	S508S (TCC→TCT)	yghK	glycolate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/48);  total (0/48)

TTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCT  >  W3110S.gb/3118361‑3118422                                                    |           ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:67576/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:100/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:47589/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:55314/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:57069/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:57881/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:58130/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:60026/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:60612/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:62424/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:62701/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:62885/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:46610/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:68423/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:72902/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:72908/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:74837/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:75335/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:75464/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:75948/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:77608/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:79831/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:83300/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:88085/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:29494/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:11046/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:12747/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:15280/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:19280/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:21426/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:22889/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:25002/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:29077/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:29341/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:29461/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:45633/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:30570/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:32134/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:32829/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:34940/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:35769/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:39004/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:39902/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:43812/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:44058/62‑1 (MQ=255) ttCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:4406/62‑1 (MQ=255)  tctcGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:62858/61‑1 (MQ=255)            ccaTGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGAGAAATCATCt  <  1:28268/51‑1 (MQ=38)                                                    |           TTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCT  >  W3110S.gb/3118361‑3118422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑