Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,160,537	A→G	P263P (CCT→CCC)	sufI ←	repressor protein for FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,160,537	0	A	G	100.0%	109.8 / NA	60	P263P (CCT→CCC)	sufI	repressor protein for FtsI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (60/0);  total (60/0)

CGGCGCTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCAGGCAGGAATCCCTG  >  W3110S.gb/3160490‑3160551                                                |               cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGTGCGGGCAGGAATCCCt   >  1:46317/4‑61 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCt   >  1:57827/4‑61 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCt   >  1:84213/4‑61 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCt   >  1:65048/4‑61 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:70883/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:56238/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:58969/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:59100/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:59720/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:60804/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:60973/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:62052/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:6488/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:65623/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:6684/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:6793/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:69444/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:54739/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:71869/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:74191/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:74440/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:75131/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:76243/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:84735/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:85357/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:85358/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:86577/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:86953/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:87193/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:87407/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:88485/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:35262/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:12581/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:14073/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:17557/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:17844/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:19612/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:21084/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:23267/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:252/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:25299/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:27226/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:30538/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:31281/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:33653/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:33691/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:12189/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:38266/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40064/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40132/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40271/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40420/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40760/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:40932/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:43132/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:45110/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:49894/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:53470/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCGGGCAGGAATCCCTg  >  1:54570/4‑62 (MQ=255) cgacgcTCGCCCGGTGCCAGCGAAAGTTGCTTCACAGATACAGGAGCGGGCAGGAATCCCTg  >  1:37602/4‑62 (MQ=255)                                                |               CGGCGCTCGCCCGGTGCCAGCGAAAGTTGCTTCACCGATACAGGAGCAGGCAGGAATCCCTG  >  W3110S.gb/3160490‑3160551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑