Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,160,822	G→A	S168S (AGC→AGT)	sufI ←	repressor protein for FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,160,822	0	G	A	100.0%	42.0 / NA	26	S168S (AGC→AGT)	sufI	repressor protein for FtsI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/0);  total (26/0)

CCATAATGGTTGGGGATAGGCAGCGACTTGCTGACT  >  W3110S.gb/3160793‑3160828                              |       ccATAATGGTTGGGGATAGGGAGCGATTTACTGACt  >  1:1294/1‑36 (MQ=25) ccATAATGGTTGGGGATAGGCAGCGATTTACTGAc   >  1:55390/1‑35 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:86258/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:10115/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:83650/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:77187/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:71886/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:71767/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:61771/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:57441/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:57100/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:56994/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:53518/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:52293/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:47585/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:44402/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:43837/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:42417/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:42115/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:40126/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:32810/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:31680/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:3008/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:28603/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:21979/1‑36 (MQ=38) ccATAATGGTTGGGGATAGGCAGCGATTTACTGACt  >  1:19697/1‑36 (MQ=38)                              |       CCATAATGGTTGGGGATAGGCAGCGACTTGCTGACT  >  W3110S.gb/3160793‑3160828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑