Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,271,725	A→G	R296R (CGT→CGC)	garR ←	tartronate semialdehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,271,725	0	A	G	100.0%	57.8 / NA	33	R296R (CGT→CGC)	garR	tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/0);  total (33/0)

TTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTT  >  W3110S.gb/3271693‑3271741                                 |                 ttGCAATACGCCATCCGGGCACGACGTCATTAGCg                >  1:25107/1‑35 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:70484/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:55203/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:61763/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:65228/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:6537/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:66650/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:68031/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:68319/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:70241/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:53305/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:76683/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:7885/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:79834/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:80500/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:81489/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:87937/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:20784/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:50598/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:49616/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:46822/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:38700/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:38122/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:37959/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:37459/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:37377/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:33904/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:30853/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:30693/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:27553/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:26787/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCACGACGTCATTAGCGAGTAACTTCGACtt  >  1:21696/1‑49 (MQ=255) ttGCAATACGCCATCCGGGCAAGACGTCATTAGCGAGTAACTTCGACtt  >  1:68291/1‑49 (MQ=255)                                 |                 TTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTT  >  W3110S.gb/3271693‑3271741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑