Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,304,524	A→G	G383G (GGT→GGC)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,304,524	0	A	G	98.8%	158.6 / ‑4.3	85	coding (1149/1245 nt)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (84/0);  minor base . (1/0);  total (85/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTT  >  W3110S.gb/3304496‑3304558                             |                                   ccAGAATCAGCGCAATCATCGGCTTGCCGCCTCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:40553/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAAtt                    >  1:55517/1‑45 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTg            >  1:60700/1‑53 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCaaa       >  1:86757/1‑58 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGc     >  1:16470/1‑60 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:50393/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:47453/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:60014/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:58477/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:57274/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:11885/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:5537/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:53723/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:52075/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:51192/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:48728/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:50301/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:50085/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:49641/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:49327/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:47931/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:49265/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:49137/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:73483/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:85986/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:84122/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:83099/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:82391/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:80986/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:80671/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:80470/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:76220/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:74632/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:60790/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:72171/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:72077/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:71404/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:71182/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:70365/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:67673/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:66900/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:64633/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:20305/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:30770/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:29619/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:29582/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:27765/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:23914/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:23269/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:23064/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:20568/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:20543/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:456/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:18859/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:1712/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:16280/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:15467/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:15048/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:14846/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:13616/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:12332/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:10446/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:32345/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:45804/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:44587/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:44408/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:43568/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:4299/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:39662/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:38695/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:3864/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:3812/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:37370/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:366/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:36588/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:36298/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:36014/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:35907/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:34393/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:32702/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:3164/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGACAAAGCg    >  1:88309/1‑61 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCCGCCCCAGACGCGGAAAATCGGGCTGcc          >  1:65323/1‑55 (MQ=255) ccAGAATCAGCGCAATCATCGGCTTGCC‑GCCCAGACGCGGAATTTCGGGCTGCCAAAGCGtt  >  1:87862/1‑62 (MQ=255) ccAGAATCAGCGAAATCATCGGCTTGCCGCCCCAGACGCGGAATTTCGGGCTGCCAAAGCg    >  1:18487/1‑61 (MQ=255)                             |                                   CCAGAATCAGCGCAATCATCGGCTTGCCACCCCAGACGCGGAATTTCGGGCTGCCAAAGCGTT  >  W3110S.gb/3304496‑3304558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑