Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,317,256	C→T	E42E (GAG→GAA)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,317,256	0	C	T	100.0%	155.2 / NA	86	E42E (GAG→GAA)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/86);  total (0/86)

TGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTT  >  W3110S.gb/3317210‑3317271                                               |                tgtCGAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:1700/62‑1 (MQ=38) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:66181/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:70454/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:70345/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:70147/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:69138/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:68804/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:68022/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:67543/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:67432/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:674/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:66709/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:71840/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:64792/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:63941/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:63616/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:62927/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:58099/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:54595/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:53839/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:5366/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:526/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:78934/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:8785/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:87276/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:867/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:86565/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:86252/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:86190/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:83585/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:7956/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:79243/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:13215/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:78117/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:76888/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:75567/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:73340/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:72995/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:72993/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:72916/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:72748/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:72288/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:20590/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:2984/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:25536/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:2440/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:24234/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:2187/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:13335/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:20776/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:20619/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:25466/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:19430/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:18335/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:18074/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:17682/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:17473/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:15647/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:14538/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:31279/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:49765/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:47682/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:4535/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:44858/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:44678/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:44676/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:44424/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:39698/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:38598/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:35810/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:35234/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:35128/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:32331/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:30562/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTCCTTGTTCATAtttt  <  1:42157/62‑1 (MQ=255) tgtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGGCGATTTCTTGTTCATAtttt  <  1:14461/62‑1 (MQ=255)  gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:20737/61‑1 (MQ=255)  gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:73581/61‑1 (MQ=255)  gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:76535/61‑1 (MQ=255)  gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:49291/61‑1 (MQ=255)      aaaTCACCGCTTTTGCGATCGATCTGTACTCGAACGTCGATTTCTTGTTCATAtttt  <  1:16712/57‑1 (MQ=38)            ccGCTTTTGCGATCGATCTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:24553/51‑1 (MQ=38)                      gatcgatcTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:11143/41‑1 (MQ=25)                        tcgatcTGTAGGCGAACGTCGATTTCTTGTTCATAtttt  <  1:86090/39‑1 (MQ=25)                         cgatcTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:53594/38‑1 (MQ=25)                          gatcTGTACGCGAACGTCGATTTCTTGTTCATAtttt  <  1:21127/37‑1 (MQ=25)                                               |                TGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTT  >  W3110S.gb/3317210‑3317271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑