Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,321,780	A→G	intergenic (‑312/+147)	yhbX ← / ← leuU	predicted hydrolase, inner membrane/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,321,780	0	A	G	100.0%	112.9 / NA	59	intergenic (‑312/+147)	yhbX/leuU	predicted hydrolase, inner membrane/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/59);  total (0/59)

AGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAG  >  W3110S.gb/3321769‑3321830            |                                                   aGAAGGGAAAAGTTTATAACTAAGGTGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:58708/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:7260/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:54477/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:88440/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:59589/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:60115/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:60477/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:63095/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:67719/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:68135/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:69416/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:69783/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:72136/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:72483/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:52818/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:74534/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:75705/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:76354/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:78774/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:79317/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:79994/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:82423/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:83352/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:83668/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:85775/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:87669/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:86940/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:87414/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:37072/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:11311/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:11860/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:13849/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:14083/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:2076/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:20785/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:25078/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:25294/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:26510/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:981/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:9577/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:32959/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:36759/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:38024/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:3849/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:38990/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:40493/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:42872/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:43640/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:43936/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:44989/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:47882/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:47996/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:50128/62‑1 (MQ=255) aGAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:52739/62‑1 (MQ=255)  gAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:11514/61‑1 (MQ=255)  gAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:8626/61‑1 (MQ=255)  gAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:28990/61‑1 (MQ=255)  gAAGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:26529/61‑1 (MQ=255)    aGGGAAAAGTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAAGTACCAGCTACGAg  <  1:56536/59‑1 (MQ=255)            |                                                   AGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAG  >  W3110S.gb/3321769‑3321830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑