Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,331,391	G→A	G73G (GGC→GGT)	obgE ←	GTPase involved in cell partioning and DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,331,391	0	G	A	100.0%	65.5 / NA	39	G73G (GGC→GGT)	obgE	GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/0);  total (39/0)

CACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTG  >  W3110S.gb/3331352‑3331410                                        |                    gaCGTCTTTGCCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:33042/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCt              >  1:63067/2‑47 (MQ=37) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCt              >  1:30609/2‑47 (MQ=37) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:59601/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:43482/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:59769/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:61020/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:62380/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:62961/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:65790/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:68838/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:75326/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:78583/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:79272/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:81863/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:8323/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:84059/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:84649/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:85838/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:86277/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:8994/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:10570/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:14819/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:17167/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:20083/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:23598/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:23810/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:24315/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:28219/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:30138/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:34257/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:34358/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:35734/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:36633/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:39859/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:42579/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACCGCGCTCTg  >  1:42980/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCTGACAGCGCTCTg  >  1:5731/2‑59 (MQ=255) gaCGTCTTTACCGCGCTTACCGGTACAGTCTCGGCTTGCACCGTTCAGACCGCGCTCTg  >  1:13158/2‑59 (MQ=255)                                        |                    CACGTCTTTACCGCGCTTACCGGTACAGTCGCGGCTTGCGCCATTCTGACCGCGCTCTG  >  W3110S.gb/3331352‑3331410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑