Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,387,395	G→A	L217L (CTA→TTA)	aaeB ←	p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,387,395	0	G	A	100.0%	151.1 / NA	85	L217L (CTA→TTA)	aaeB	p‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (85/0);  total (85/0)

CAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGC  >  W3110S.gb/3387352‑3387411                                            |                 cAGCGGGAAGATTCCATATTCAGGTTGCTTCGCATACCTTGTAACGCCgtagtac       >  1:23296/1‑53 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTc    >  1:79336/1‑58 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCg   >  1:43063/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCg   >  1:62774/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCg   >  1:64758/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCg   >  1:66006/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCg   >  1:9744/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:62176/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:6685/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:87719/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:65626/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:64815/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:87995/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:62869/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:62251/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:62182/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:67299/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:61151/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:57714/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:54403/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:51177/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:49435/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:4707/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:46166/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:42676/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:87625/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:86261/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:86175/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:8608/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:85994/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:8538/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:83772/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:83091/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:8239/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:87574/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:78845/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:78068/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:77541/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:7713/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:76135/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:87692/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:71585/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:68046/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:16665/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:10892/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:22490/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:21594/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:20855/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:2080/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:19500/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:18787/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:18250/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:17277/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:17274/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:22717/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:16566/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:15790/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:153/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:1396/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:13892/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:13769/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:13655/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:13379/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:12732/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:24747/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:41885/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:41297/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:39581/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:38610/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:38537/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:38268/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:36523/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:34900/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:34122/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:31937/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:31059/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:30893/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:30760/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:2982/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:25795/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:25028/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:24184/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACCTTGTAACGACGTAGTACGTCGc  >  1:35998/1‑60 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATACATTGTAACGCCGTAGTACGTCg   >  1:42524/1‑59 (MQ=255) cAGCGGGAAGATTCCATATTCAGGTTGCTCCGCATACCTTGTAACGCCGTAGTACGTCGc  >  1:79691/1‑60 (MQ=255)                                            |                 CAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGC  >  W3110S.gb/3387352‑3387411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑