Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,394,848 A→T V422V (GTT→GTA)  yhdP ← conserved membrane protein, predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,394,8480AT100.0% 168.3 / NA 94V422V (GTT→GTA) yhdPconserved membrane protein, predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/94);  total (0/94)

GTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCC  >  W3110S.gb/3394795‑3394856
                                                     |        
gcttCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:16799/60‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGATGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:64520/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGGAAACCATTTTCTACGCTGcc  <  1:670/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:7754/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:78671/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:77264/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:75037/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:74481/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:73849/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:73798/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:70178/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:69061/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:68229/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:6595/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:62425/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:61852/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:61658/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:60981/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:60748/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:58813/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:58529/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:56013/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:11680/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:54211/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:84113/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:9573/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:9482/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:88451/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:86802/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:86786/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:85498/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:85342/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:8526/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:84903/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:84822/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:54359/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:83985/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:8343/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:83276/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:82765/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:81901/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:81874/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:8186/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:80519/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:80416/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:80182/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:25460/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:33998/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:3355/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:33207/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:33024/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:32393/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:32311/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:30224/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:30128/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:28777/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:26361/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:53210/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:23500/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:2227/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:20205/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:18977/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:18476/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:15431/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:15377/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:14914/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:13242/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:1256/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:36257/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:53462/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:52942/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:5285/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:52458/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:52239/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:4774/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:47241/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:47078/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:44762/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:42941/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:42369/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:4231/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:39062/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:38739/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:38516/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:38467/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:37891/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:37469/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:34366/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGAAAGCAAACCATTTTCTACGCTGcc  <  1:13622/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTTGCCTGCTTCATCGACCCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:69920/62‑1 (MQ=255)
gTTTCGTAAGGCATCTTAGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:8949/62‑1 (MQ=255)
 tttCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:24138/61‑1 (MQ=255)
 tttCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:69588/61‑1 (MQ=255)
 tttCGTAAGGCATCTTTGCCTGCTTCATCGACGCAGTAAGCAAACCATTTTCTACGCTGcc  <  1:77600/61‑1 (MQ=255)
                                                     |        
GTTTCGTAAGGCATCTTTGCCTGCTTCATCGACGCGGTAAGCAAACCATTTTCAACGCTGCC  >  W3110S.gb/3394795‑3394856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑