Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,402,432 | T→A | V252V (GTA→GTT) | yhdA ← | conserved inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,402,432 | 0 | T | A | 98.8% | 143.5 / NA | 81 | V252V (GTA→GTT) | yhdA | conserved inner membrane protein |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (80/0); minor base C (1/0); total (81/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGAT > W3110S.gb/3402408‑3402469 | catcatcaCGATCCCGTGTGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:19066/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCCACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:42073/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAGCGTTGCTAACTGAt > 1:1226/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAAc > 1:67402/1‑49 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTg > 1:10131/1‑60 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGag > 1:61291/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGa > 1:54748/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGa > 1:85930/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGa > 1:31329/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGa > 1:77980/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGa > 1:27904/1‑61 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:64062/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:53830/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:66911/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:66798/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:65885/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:65022/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:64372/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:9751/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:59624/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:59345/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:58596/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:55229/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:54982/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:54496/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:79479/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:970/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:9490/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:88312/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:87497/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:85382/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:84566/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:81879/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:8/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:70853/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:79234/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:7861/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:77526/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:76275/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:74760/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:71826/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:71657/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:71531/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:22973/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:53772/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:35700/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:28617/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:28165/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:27943/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:27837/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:27681/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:27433/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:2584/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:36235/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:21628/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:21532/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:17464/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:17367/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:17163/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:17060/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:13256/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:13044/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:48054/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:53054/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:52728/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:52662/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:52470/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:52439/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:52048/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:50938/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:48916/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:39885/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:47663/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:47656/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:46415/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:43848/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:43095/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:41313/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:40858/1‑62 (MQ=255) catcatcaCGATCCCGTGGGTACAAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:44638/1‑62 (MQ=255) cataatcaCGATCCCGTGGGTACCAACTTTCTCTTGATCTTCCAGTAACGTTGCTAACTGAt > 1:9670/1‑62 (MQ=255) | CATCATCACGATCCCGTGGGTACCTACTTTCTCCTGATCTTCCAGTAACGTTGCTAACTGAT > W3110S.gb/3402408‑3402469 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |