Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,406,971	C→T	A400A (GCC→GCT)	accC →	acetyl‑CoA carboxylase, biotin carboxylase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,406,971	0	C	T	100.0%	78.1 / NA	45	A400A (GCC→GCT)	accC	acetyl‑CoA carboxylase, biotin carboxylase subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/45);  total (0/45)

ATTGCCCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGA  >  W3110S.gb/3406966‑3407027      |                                                         aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCTACGGTATCAAAACCAACGTTGa  <  1:32687/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:67296/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:47939/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:48963/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:49622/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:50107/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:50815/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:5835/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:58648/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:61865/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:62345/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:63793/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:43924/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:69562/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:70586/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:70800/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:76704/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:78942/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:80082/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:81011/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:83665/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:85365/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:87457/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:25949/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:10122/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:10864/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:11865/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:12918/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:1530/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:16532/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:18489/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:19681/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:22694/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:23104/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:45783/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:27031/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:27800/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:29483/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:29868/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:32409/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:37009/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:39602/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:40558/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:43174/62‑1 (MQ=255) aTTGCTCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGa  <  1:10011/62‑1 (MQ=255)      |                                                         ATTGCCCGCATGAAGAATGCGCTGCAGGAGCTGATCATCGACGGTATCAAAACCAACGTTGA  >  W3110S.gb/3406966‑3407027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑