Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,431,424	A→G	P225P (CCA→CCG)	purD →	phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,431,424	0	A	G	100.0%	114.6 / NA	60	P225P (CCA→CCG)	purD	phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/60);  total (0/60)

TACCGGACCAAACACCGGCGGGATGGGCGCTTACTCCCCCGCGCCGGTAGTAACCGATGACG  >  W3110S.gb/3431415‑3431476          |                                                     tACCGGGCCGAACTCCGGCGGGATGGGCGCTTTCTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:60078/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:60908/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:49166/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:14020/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:51035/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:51270/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:51630/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:52610/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:54228/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:56977/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:57090/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:57820/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:57868/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:59282/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:49752/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:6112/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:64127/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:66606/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:6733/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:68647/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:71751/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:73078/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:73491/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:81889/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:83535/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:85586/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:86058/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:9455/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:47476/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:14355/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:1448/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:16571/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:17771/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:20247/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:20902/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:23391/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:24866/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:25822/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:26594/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:27463/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:27848/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:30482/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:41209/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:48907/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:47310/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:47015/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:46914/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:45862/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:45631/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:43309/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:31599/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:39674/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:38907/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:36327/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:35060/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:33724/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGAGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:61748/62‑3 (MQ=255) tACCGGGCCGAACACCGGCGGGATGGGAGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:61641/62‑3 (MQ=255)  aCCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:62370/61‑3 (MQ=255)  aCCGGGCCGAACACCGGCGGGATGGGCGCTTACTCCCCTGCGCCAGTAGTGACCGATGagg  <  1:8847/61‑3 (MQ=255)          |                                                     TACCGGACCAAACACCGGCGGGATGGGCGCTTACTCCCCCGCGCCGGTAGTAACCGATGACG  >  W3110S.gb/3431415‑3431476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑