Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,432,705	A→G	T219T (ACT→ACC)	zraR ←	fused DNA‑binding response regulator in two‑component regulatory system with ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,432,705	0	A	G	100.0%	97.8 / NA	54	T219T (ACT→ACC)	zraR	fused DNA‑binding response regulator in two‑component regulatory system with ZraS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/0);  total (54/0)

GGCGTTTATCGGCTCCAGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGATTCCAGCAAG  >  W3110S.gb/3432689‑3432750                 |                                              ggCGTTTGTCGGCCCCGGTATACGCCCCTTTTTCGTg                           >  1:79644/1‑37 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGt                            >  1:61730/1‑36 (MQ=18) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGGCCGAACAATTCAGACTCCAGCAAg  >  1:85397/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGCCCGCACAATTCAGCCTCCAGCa    >  1:2572/1‑60 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGCCCGAACAATTCAGACTCCAGCAAg  >  1:30620/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCa              >  1:14949/1‑50 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCaa   >  1:48882/1‑61 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCaa   >  1:76015/1‑61 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCa    >  1:65731/1‑60 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:6137/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:67001/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:64604/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:62911/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:75840/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:6101/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:59997/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:59770/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:5889/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:67047/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:74192/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:75046/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:50590/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:75929/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:76233/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:76990/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:78866/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:81026/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:81678/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:86847/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:32789/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:12329/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:1682/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:17342/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:21821/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:22142/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:23599/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:24139/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:25105/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:26604/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:29976/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:3245/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:32462/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:53172/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:37510/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:37955/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:43276/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:43617/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:44757/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:45091/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:47340/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:4973/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:50468/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:10660/1‑62 (MQ=25) ggCGTTTGTCGGCCCCGGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGACTCCAGCAAg  >  1:50622/1‑62 (MQ=25)                 |                                              GGCGTTTATCGGCTCCAGTAAACGCCCCTTTTTCGTGACCGAACAATTCAGATTCCAGCAAG  >  W3110S.gb/3432689‑3432750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑