Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,444,986	C→T	A58A (GCC→GCT)	thiH →	thiamin biosynthesis ThiGH complex subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,444,986	0	C	T	100.0%	125.7 / NA	68	A58A (GCC→GCT)	thiH	thiamin biosynthesis ThiGH complex subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (68/0);  total (68/0)

CCTGCCGCCAGTGGCTATCTGGAACAACTGGCCCAACGGGCGCAGCGTCTGACCCGTCAGCG  >  W3110S.gb/3444954‑3445015                                 |                              ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCaa                            >  1:74986/1‑36 (MQ=16) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTgcc           >  1:25056/1‑51 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGAc           >  1:53460/1‑53 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCg        >  1:40422/1‑56 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAg    >  1:8806/1‑60 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:63282/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:71922/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:70601/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:69940/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:69427/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:65151/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:65147/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:72814/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:61482/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:59844/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:58555/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:58289/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:58129/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:57452/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:5560/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:72639/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:9797/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:73326/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:75652/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:7621/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:76998/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:77805/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:79/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:80165/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:81016/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:81059/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:8179/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:82341/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:83499/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:84671/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:10701/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:31064/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:10819/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:10913/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:11015/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:12779/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:1309/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:15328/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:17245/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:18553/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:22212/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:2310/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:2438/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:25062/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:27842/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:28262/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:53498/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:31880/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:32717/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:33627/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:35374/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:39151/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:45734/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:4668/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:4696/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:4716/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:47325/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:48525/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:51655/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:51678/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:52399/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCGTCTGACCCGTCAGCg  >  1:53296/1‑62 (MQ=255) ccTGCCGCCAGTGAATACCTGGAACCACTGGCTCAACGGGCGCAGCCTCTGACCCGTCAGCg  >  1:27224/1‑62 (MQ=255)                                 |                              CCTGCCGCCAGTGGCTATCTGGAACAACTGGCCCAACGGGCGCAGCGTCTGACCCGTCAGCG  >  W3110S.gb/3444954‑3445015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑