Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,497,707	T→C	L21P (CTG→CCG)	gldA →	glycerol dehydrogenase, NAD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,497,707	0	T	C	100.0%	23.8 / NA	15	L21P (CTG→CCG)	gldA	glycerol dehydrogenase, NAD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/0);  total (15/0)

GGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAG  >  W3110S.gb/3497684‑3497745                        |                                       gggCGCTGATGTGATTAATTGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:62844/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAGTACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:3660/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTg                           >  1:61702/1‑37 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGATGCCGCTGGCAGAACGCTGGTTAg  >  1:85315/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGcc                      >  1:5783/1‑42 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:16194/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:17809/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:24001/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:42611/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:57315/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:74752/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:81719/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:84650/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:9073/1‑62 (MQ=255) gggCGCTGATGTGATTAATCGTCCGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAg  >  1:9484/1‑62 (MQ=255)                        |                                       GGGCGCTGATGTGATTAATCGTCTGGGCGAATACCTGAAGCCGCTGGCAGAACGCTGGTTAG  >  W3110S.gb/3497684‑3497745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑