Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,531,163	C→T	D51D (GAC→GAT)	cpxR →	DNA‑binding response regulator in two‑component regulatory system with CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,531,163	0	C	T	100.0%	46.2 / NA	28	D51D (GAC→GAT)	cpxR	DNA‑binding response regulator in two‑component regulatory system with CpxA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/28);  total (0/28)

TGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGACGTAATGA  >  W3110S.gb/3531109‑3531170                                                       |        tcgggAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:46858/60‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:44596/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:9293/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:80204/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:71727/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:62403/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:61269/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:59973/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:59412/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:51559/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:47916/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:46389/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:12083/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:39914/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:36750/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:36118/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:3307/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:32099/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:30710/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:28796/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:2645/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:24254/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:21019/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:16843/62‑1 (MQ=255) tGGGGAACAGGCGCTTGATCATCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:14474/62‑1 (MQ=255)  ggggAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTTATGTAatga  <  1:67469/61‑1 (MQ=255)  ggggAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:23765/61‑1 (MQ=255)  ggggAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGATGTAatga  <  1:86772/61‑1 (MQ=255)                                                       |        TGGGGAACAGGCGCTTGATCTTCTGGACGACAGCATTGATTTACTTTTGCTTGACGTAATGA  >  W3110S.gb/3531109‑3531170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑