Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,541,004	T→C	D102D (GAT→GAC)	rhaA →	L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,541,004	0	T	C	100.0%	56.6 / NA	31	D102D (GAT→GAC)	rhaA	L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/0);  total (31/0)

GCCATCTATCTGGAATCAGATACGCCAGTCTCGCGCGACCAGATCAAACCAGAGCACTTCA  >  W3110S.gb/3540984‑3541044                     |                                         gCCATCTATCTGGAATCAGCCACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:30955/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTcgcgcgcgc                         >  1:82045/1‑37 (MQ=25) gCCATCTATCTGGAATCAGACACGCCCGTcgcgcgcg                          >  1:71679/1‑37 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGa                         >  1:43870/1‑38 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGCCCAGATCAAACCag           >  1:28910/1‑52 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGAcc                       >  1:81996/1‑40 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGAcc                       >  1:39819/1‑40 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGAcc                       >  1:69/1‑40 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAg                     >  1:32456/1‑42 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCaaa               >  1:8663/1‑48 (MQ=37) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCaaa               >  1:11531/1‑48 (MQ=37) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCagag         >  1:67708/1‑54 (MQ=38) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCagag         >  1:4432/1‑54 (MQ=38) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCag           >  1:87888/1‑52 (MQ=38) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:63976/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:45729/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:72526/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:80450/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:86851/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:61614/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:60654/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:56430/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:49946/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:45547/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:42699/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:39166/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:34772/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:33884/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:21618/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:20025/1‑61 (MQ=255) gCCATCTATCTGGAATCAGACACGCCCGTCGCGCGCGACCAGATCAAACCAGAGCACTTCa  >  1:13261/1‑61 (MQ=255)                     |                                         GCCATCTATCTGGAATCAGATACGCCAGTCTCGCGCGACCAGATCAAACCAGAGCACTTCA  >  W3110S.gb/3540984‑3541044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑