Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,558,354	C→T	Q293Q (CAG→CAA)	yiiD ←	predicted acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,558,354	0	C	T	100.0%	82.5 / NA	46	Q293Q (CAG→CAA)	yiiD	predicted acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/0);  total (46/0)

AGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCGACCTG  >  W3110S.gb/3558295‑3558356                                                            |   aGAACGATATACGTTCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:61667/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:65618/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:4877/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:51532/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:53664/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:55121/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:56258/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:56358/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:5698/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:5708/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:5809/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:61710/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:48691/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:66211/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:66771/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:74777/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:75910/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:79086/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:81152/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:82372/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:83825/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:86855/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:87071/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:28790/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:10767/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:12005/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:13652/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:14537/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:19223/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:22287/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:22309/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:22976/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:2550/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:28096/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:28374/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:10138/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:30195/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:32697/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:34134/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:34665/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:34993/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:39524/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:40670/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:44877/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:4672/1‑62 (MQ=255) aGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCTACTTg  >  1:46766/1‑62 (MQ=255)                                                            |   AGAACGATATACGTGCCTTCAAACACTGCACCCGGCGTCTCGTCGCCAAAGATTTCGACCTG  >  W3110S.gb/3558295‑3558356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑