Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,588,033	A→C	L561M (CTT→TTG)	polA ←	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,588,033	0	A	C	100.0%	26.6 / NA	17	L561M (CTT→TTG)	polA	fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/17);  total (0/17)

CTCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGAAGGGTGAG  >  W3110S.gb/3587988‑3588041                                              |         ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:59869/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:84080/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:80916/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:77754/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:67635/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:65789/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:63406/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:62640/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:61382/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:12022/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:54879/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:52994/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:44208/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:43654/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:40077/53‑1 (MQ=38) ttCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:3817/53‑1 (MQ=38)           aTTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGCAAGGTGAg  <  1:23659/44‑1 (MQ=37)                                              |         CTCACCTGCAATTTCATGCGCTTTCTTTTCCAGCTCAGCCAGACGAAGGGTGAG  >  W3110S.gb/3587988‑3588041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑