Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,668,398	C→A	G123V (GGT→GTT)	rfe ←	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,668,398	0	C	A	100.0%	24.5 / NA	17	G123V (GGT→GTT)	rfe	UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/0);  total (17/0)

CCAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAA  >  W3110S.gb/3668356‑3668415                                           |                  ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTga       >  1:16561/1‑55 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:44733/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:81832/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:74312/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:70647/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:65891/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:64432/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:57119/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:51290/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:46259/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:35131/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:31049/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:25209/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:17755/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATaa  >  1:17065/1‑60 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACTGAGATa   >  1:42096/1‑59 (MQ=255) ccAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAAACCAGGCTACAGAGATaa  >  1:42636/1‑60 (MQ=255)                                           |                  CCAAACGGTCCGAGCACCATCTCCCAGGAGCCAAAGATATAACCCAGGCTACTGAGATAA  >  W3110S.gb/3668356‑3668415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑