Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,709,955	T→C	L92L (CTT→CTC)	asnC →	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,709,955	0	T	C	100.0%	97.0 / NA	53	L92L (CTT→CTC)	asnC	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/53);  total (0/53)

TATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAG  >  W3110S.gb/3709901‑3709962                                                       |        tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCGAAGCTGGAAAGCCTCGATGAAg  <  1:1826/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:75554/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:4454/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:46295/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:46344/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:476/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:48938/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:53332/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:53990/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:54675/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:57875/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:60327/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:61712/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:67480/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:11239/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:76423/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:78953/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:80764/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:81032/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:82048/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:82988/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:85201/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:85518/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:86011/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:87833/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:38449/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:12757/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:12911/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:13512/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:14092/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:1644/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:16585/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:16833/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:2026/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:12052/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:21149/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:11412/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:21854/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:26080/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:27633/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:29597/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:30792/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:10445/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:31581/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:33200/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:33386/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:36309/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:36713/62‑1 (MQ=255) tataTTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGAAAAGCCTCGATGAAg  <  1:59625/62‑1 (MQ=255)  atatTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:44494/61‑1 (MQ=255)  atatTAAAGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:71309/61‑1 (MQ=255)       aaaGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:21362/56‑1 (MQ=255)       aaaGAGCGCCAAAGACTATCCTTCCGCGCTGGCAAAGCTGGAAAGCCTCGATGAAg  <  1:52423/56‑1 (MQ=255)                                                       |        TATATTAAAGAGCGCCAAAGACTACCCTTCCGCGCTGGCAAAGCTGGAAAGCCTTGATGAAG  >  W3110S.gb/3709901‑3709962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑