Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,771,447	G→A	G103S (GGC→AGC)	yidR →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,771,447	0	G	A	100.0%	32.5 / NA	20	G103S (GGC→AGC)	yidR	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/20);  total (0/20)

GAAACATGGCATTACGACTTCCATCATCGGCGCGGAGTGATTGTTGAAGGCGGCAAGATGAG  >  W3110S.gb/3771399‑3771460                                                 |              gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:56375/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:86169/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:84412/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:76349/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:67288/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:66745/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:65878/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:59515/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:57838/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:15964/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:54609/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:44919/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:21895/62‑1 (MQ=255) gaAACATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:19496/62‑1 (MQ=255)  aaaCTTGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:1910/61‑1 (MQ=255)  aaaCATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:462/61‑1 (MQ=255)  aaaCATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:35501/61‑1 (MQ=255)  aaaCATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:32798/61‑1 (MQ=255)  aaaCATGGTATTACGACTTTCATCATCGGCGCGGAGTGATTGTTGAAAGCGGCAAGGTGAg  <  1:74432/61‑1 (MQ=255)   aaCATGGTATTACGACTTTCATCATCGGCGCGGAGTTATTGTTGAAAGCGGCAAGGTGAg  <  1:2392/60‑1 (MQ=255)                                                 |              GAAACATGGCATTACGACTTCCATCATCGGCGCGGAGTGATTGTTGAAGGCGGCAAGATGAG  >  W3110S.gb/3771399‑3771460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑