Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,804,808	C→A	G107G (GGC→GGA)	yicJ →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,804,808	0	C	A	100.0%	179.8 / NA	99	G107G (GGC→GGA)	yicJ	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (99/0);  total (99/0)

ATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGA  >  W3110S.gb/3804754‑3804815                                                       |        cttCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:26848/2‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAAt    >  1:25354/1‑60 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATg   >  1:44142/1‑61 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATg   >  1:72041/1‑61 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATg   >  1:39792/1‑61 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATg   >  1:31877/1‑61 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATg   >  1:3705/1‑61 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:584/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:67489/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:65681/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:65026/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:63544/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:61901/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:61344/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:6118/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:60951/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:59593/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:6828/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:57619/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:5736/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:5672/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:55773/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:55217/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:5372/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:52366/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:48978/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:48940/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:46416/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:77164/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:9348/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:87956/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:87123/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:85563/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:8375/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:82708/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:82534/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:78624/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:78432/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:77973/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:77555/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:77538/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:67556/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:76817/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:7676/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:75291/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:74227/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:73048/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:72870/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:72563/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:71403/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:68382/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:42476/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:6767/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:22268/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:46298/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27401/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27073/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:26727/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:26401/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:25750/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:24259/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:2302/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:22952/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:22802/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:22756/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27436/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:21221/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:18863/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:17945/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:1717/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:16982/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:1615/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:15551/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:15521/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:14374/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:13239/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:34897/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:45189/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:10097/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:42192/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:4211/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:40473/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:3887/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:38298/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:3771/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:36208/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:36176/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27472/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:33084/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:32610/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:32604/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:29558/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:28987/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:28759/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:28683/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:28494/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27986/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGAAAAATGa  >  1:27867/1‑62 (MQ=255) aTTCGGGATCGTCTGTGTACTGGCCTATAGCACCCCAGATCTCAGTATGAACGGAAAAATGa  >  1:21657/1‑62 (MQ=255)                                                       |        ATTCGGGATCGTCTGTGTACTGGCCTATAGCACGCCAGATCTCAGTATGAACGGCAAAATGA  >  W3110S.gb/3804754‑3804815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑