Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,830,083	C→A	intergenic (+9/+30)	mutM → / ← coaD	formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/pantetheine‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,830,083	0	C	A	100.0%	47.6 / NA	29	intergenic (+9/+30)	mutM/coaD	formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase/pantetheine‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/0);  total (29/0)

CGGCAGTGCCAGAAGTAATTCATGCGCGCCGGATGGCATACCATCCGGCATAAACGCTACG  >  W3110S.gb/3830057‑3830117                           |                                   cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:50846/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:9385/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:87673/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:87504/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:74579/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:72264/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:69770/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:67377/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:66195/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:65388/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:64830/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:63485/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:62970/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:55300/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:53926/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:16509/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:50226/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:4930/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:48060/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:47139/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:41537/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:37621/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:36127/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:29156/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:27174/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:2390/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:21668/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:21018/1‑61 (MQ=255) cGGCAGTGCCAGAAGTAATTGATGCGAGCCGGATGGCATACCATCCGGCATAAacgctacg  >  1:17302/1‑61 (MQ=255)                           |                                   CGGCAGTGCCAGAAGTAATTCATGCGCGCCGGATGGCATACCATCCGGCATAAACGCTACG  >  W3110S.gb/3830057‑3830117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑