Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,845,387	A→G	D34D (GAT→GAC)	rfaF ←	ADP‑heptose:LPS heptosyltransferase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,845,387	0	A	G	100.0%	98.9 / NA	54	D34D (GAT→GAC)	rfaF	ADP‑heptose:LPS heptosyltransferase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/0);  total (54/0)

ATCACATCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGACAT  >  W3110S.gb/3845382‑3845443      |                                                         aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCg                        >  1:26364/1‑40 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGAc            >  1:13202/1‑52 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGa     >  1:29923/1‑59 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:79302/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:57060/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:58320/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:6254/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:64041/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:64524/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:6481/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:67226/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:69445/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:72657/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:7393/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:76443/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:77119/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:7887/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:54287/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:7970/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:80138/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:80239/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:80572/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:83074/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:8367/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:84133/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:85413/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:85700/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:9340/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:50675/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:11388/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:12410/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:14529/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:17209/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:17303/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:19662/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:243/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:24749/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:27794/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:32506/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:32827/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:35471/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:36498/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:38161/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:3830/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:43089/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:44093/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:45350/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:46821/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:49423/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAcat  >  1:11108/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAca   >  1:76999/1‑61 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAca   >  1:32780/1‑61 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGAaat  >  1:25274/1‑62 (MQ=255) aTCACGTCGATTATCGCCTGGGGATAGCACGCCTGGAGCGTGCGATAGAGACTTTGCGAca   >  1:82985/1‑61 (MQ=255)      |                                                         ATCACATCGATTATCGCCTGGGGATAGCGCGCCTGGAGCGTGCGATAGAGACTTTGCGACAT  >  W3110S.gb/3845382‑3845443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑