Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,867,165	A→T	A324A (GCT→GCA)	mtlA ←	fused mannitol‑specific PTS enzyme IIABC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,867,165	0	A	T	100.0%	163.6 / NA	90	A324A (GCT→GCA)	mtlA	fused mannitol‑specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/90);  total (0/90)

AGCAGAGACAACGAAGGAGACAGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAG  >  W3110S.gb/3867144‑3867205                      |                                         tgCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65013/61‑1 (MQ=255) ggCATAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:64087/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGGACACACACCCGCGATGTTAGCGAAGTAAg  <  1:59249/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56303/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65134/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:64498/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:62359/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:58857/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:58010/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:57088/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56320/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:65759/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:56250/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:55944/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:55924/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:5521/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:54583/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:53026/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:52342/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:51753/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:50135/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:11027/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:77222/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:8829/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:87514/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85892/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85879/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:85285/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:8255/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:81308/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78913/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78447/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:78299/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:49060/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:74208/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:73342/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:73045/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:71649/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:71622/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:70804/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:69898/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:69759/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:67174/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:66147/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18754/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:48339/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:28449/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:27445/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:25812/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:25335/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23988/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23618/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:23419/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:19330/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18824/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:29598/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:18040/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:16805/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15841/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15817/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15668/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15258/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14943/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14579/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:14188/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:11488/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:40548/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:47920/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:47835/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:44824/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:44744/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:43680/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:42722/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:42302/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:40979/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:31123/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3908/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:39019/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38993/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38673/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:38486/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:36929/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:36318/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3474/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:3360/61‑1 (MQ=255) ggCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGAGAAGTAAg  <  1:8708/61‑1 (MQ=255)  gCAGAGACAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:33348/61‑1 (MQ=255)       gaCAACGAAGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:51895/56‑1 (MQ=255)              aaGGAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:35942/49‑1 (MQ=255)                ggAGACTGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAg  <  1:15474/47‑1 (MQ=255)                      |                                         AGCAGAGACAACGAAGGAGACAGCCATCGCCGCACACACACCCGCGATGTTAGCGAAGTAAG  >  W3110S.gb/3867144‑3867205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑