Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,885,091	C→T	L396L (CTG→TTG)	aldB →	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,885,091	0	C	T	100.0%	75.7 / NA	42	L396L (CTG→TTG)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/0);  total (42/0)

CTGGAAGGTGAACTGAAAGACGGCTACTACCTCGAACCGACGATTCTGTTTGGTCAGAACAA  >  W3110S.gb/3885046‑3885107                                              |                 ctgGAAGGCGAACTGAAAGACGGCTGCTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:65220/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAAGCGACGATTTTGTTTGGTCAGAACaa  >  1:24519/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGCCGATTTTGTTTGGTCAGAACaa  >  1:56932/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACat  >  1:72839/1‑61 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:10770/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:51905/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:53212/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:54216/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:54318/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:56385/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:56970/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:59729/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:63637/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:64813/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:70115/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:70311/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:71171/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:7291/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:75315/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:78216/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:84010/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:24286/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:10883/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:15672/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:17023/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:17241/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:20443/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:20958/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:20986/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:51261/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:2672/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:2870/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:28848/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:36233/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:3977/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:40801/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:46755/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACaa  >  1:50277/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACCGACGATTTTGTTTGGTCAGAACa   >  1:25032/1‑61 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAACAGACGATTTTGTTTGGTCAGAACaa  >  1:12123/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACCTCGAAACGACGATTTTGTTTGGTCAGAACaa  >  1:66561/1‑62 (MQ=255) ctgGAAGGCGAACTGAAAGACGGCTACTACATCGAACCGACGATTTTGTTTGGTCAGAACa   >  1:68166/1‑61 (MQ=255)                                              |                 CTGGAAGGTGAACTGAAAGACGGCTACTACCTCGAACCGACGATTCTGTTTGGTCAGAACAA  >  W3110S.gb/3885046‑3885107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑