Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,938,807 C→T A350T (GCT→ACT)  yhjV ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,938,8070CT100.0% 195.7 / NA 107A350T (GCT→ACT) yhjVpredicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/107);  total (0/107)

Only 100 of 107 total aligned reads displayed.

ACACCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGCGCAG  >  W3110S.gb/3938750‑3938811
                                                         |    
acacTAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:65523/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAATGTAATGACGATAAAAGTGCAg  <  1:60996/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:5637/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:65346/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:63906/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:61988/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:60707/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:60264/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:59089/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:58483/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:57742/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:57658/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:57620/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66001/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:55909/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:55683/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:53330/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:49714/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:49253/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:48788/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:48546/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:46021/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:45646/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:1070/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:45045/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:77217/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:88472/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:8826/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:85232/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:84691/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:8445/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:83784/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:83253/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:80805/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:80587/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:80113/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:77384/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:45291/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:77168/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:76804/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:73963/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:73544/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:72809/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:71877/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:68662/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66856/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66582/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66222/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66129/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:21672/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:30582/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:29398/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:27146/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:26408/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:2607/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:24304/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:2416/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:23338/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:22628/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:22215/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:22121/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:44878/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:1943/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:19205/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:16798/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:16284/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:15103/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:14363/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:12958/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:12540/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:12476/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:12182/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:11149/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:36532/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:44946/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:44686/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:44539/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:43262/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:41179/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:40229/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:38648/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:37980/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:37146/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:3686/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:36579/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:31522/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:35593/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:3374/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:33533/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:33361/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:3246/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:31731/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:31639/62‑1 (MQ=255)
acacCAGCACCGATACACGAAACGAAACCAAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:67802/62‑1 (MQ=255)
 cacCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:32032/61‑1 (MQ=255)
    cAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:55996/58‑1 (MQ=255)
          cGATACACTAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:29813/52‑1 (MQ=255)
           gATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:44634/51‑1 (MQ=255)
             tACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:66761/49‑1 (MQ=255)
                 cgaaacgaaaCCCAAATCGTCAACGTAATGACGATAAAAGTGCAg  <  1:28039/45‑1 (MQ=255)
                                                         |    
ACACCAGCACCGATACACGAAACGAAACCCAAATCGTCAACGTAATGACGATAAAAGCGCAG  >  W3110S.gb/3938750‑3938811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑