Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,971,456	T→C	P76P (CCT→CCC)	yhjA →	predicted cytochrome C peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,971,456	0	T	C	100.0%	123.4 / NA	64	P76P (CCT→CCC)	yhjA	predicted cytochrome C peroxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (64/0);  total (64/0)

GCAGAATTACCCGCCTATTATTATATTCCTGGCGCGAAACAGTTGATGGATTACGACATTAA  >  W3110S.gb/3971427‑3971488                              |                                 gCAGAATTATCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:63744/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGgcgc                             >  1:84301/1‑35 (MQ=38) gCAGAATTACCTGCCTATTATTATATTCCCGgcgc                             >  1:45849/1‑35 (MQ=38) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGaaa                         >  1:36033/1‑39 (MQ=38) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGATACAGTTGATGGATTACGACATaaa  >  1:7404/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACAtaa   >  1:1113/1‑59 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACAtaa   >  1:66903/1‑59 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACAtaa   >  1:81907/1‑59 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACAtaa   >  1:33305/1‑59 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:59675/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:63346/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:63213/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:61212/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:60989/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:66425/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:59661/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:55375/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:54107/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:53684/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:6376/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:66011/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:47362/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:67278/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:68844/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:69332/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:70971/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:71621/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:76306/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:77266/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:8203/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:83058/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:84953/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:86062/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:9885/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:26678/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:1130/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:11307/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:13990/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:14239/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:14656/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:15244/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:16457/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:17494/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:20049/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:21182/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:22905/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:24815/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:25411/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:25850/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:53374/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:28431/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:30087/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:3079/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:31228/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:31868/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:32225/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:32231/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:32662/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:35632/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:40187/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:43024/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:44436/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:47322/1‑62 (MQ=255) gCAGAATTACCTGCCTATTATTATATTCCCGGCGCGAAACAGTTGATGGATTACGACATaaa  >  1:50139/1‑62 (MQ=255)                              |                                 GCAGAATTACCCGCCTATTATTATATTCCTGGCGCGAAACAGTTGATGGATTACGACATTAA  >  W3110S.gb/3971427‑3971488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑