Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,068,888	G→A	T658T (ACG→ACA)	glgB →	1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,068,888	0	G	A	100.0%	64.4 / NA	38	T658T (ACG→ACA)	glgB	1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/0);  total (38/0)

AAAGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACGCCGGTACCGCGTCATGATTATCG  >  W3110S.gb/4068850‑4068911                                       |                        aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTAtag  >  1:79525/1‑60 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:68874/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:52929/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:55140/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:55494/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:56084/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:58746/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:60237/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:63119/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:63431/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:65146/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:51093/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:726/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:73706/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:74830/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:75930/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:80622/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:81884/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:83758/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:49609/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:12631/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:19900/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:21329/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:21897/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:24879/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:25156/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:30367/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:31629/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:34803/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:35273/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:37156/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:41306/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:42886/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:44637/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:46284/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGCGTCATGATTATCg  >  1:10140/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACACCGGTGCCGAGTCATGATTATCg  >  1:34857/1‑62 (MQ=255) aaaGAGGGTAACGAAATCATCGTTGCCAGTAACCTTACACCGGTGCCGCGTCATGATTATCg  >  1:42313/1‑62 (MQ=255)                                       |                        AAAGAGGGTAACGAAATCATCGTTGCCAGTAACTTTACGCCGGTACCGCGTCATGATTATCG  >  W3110S.gb/4068850‑4068911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑