Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,080,432	T→C	D207D (GAT→GAC)	glpR →	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,080,432	0	T	C	100.0%	82.1 / NA	44	D207D (GAT→GAC)	glpR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/0);  total (44/0)

TCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCG  >  W3110S.gb/4080378‑4080438                                                       |       tCGCACCTAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTca  >  1:80343/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGCCCACTc   >  1:76143/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACt    >  1:37755/1‑59 (MQ=39) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:70891/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:51517/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:55215/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:56853/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:58096/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:58138/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:60192/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:6292/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:66884/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:67929/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:68866/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:46336/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:74503/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:75086/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:78240/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:78848/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:79833/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:80449/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:88169/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:8990/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:24364/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:10472/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:11813/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:1365/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:1381/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:18186/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:18278/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:18980/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:19442/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:10331/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:24757/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:25784/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:28357/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:3107/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:35991/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:36901/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:37630/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:43186/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGACCACTc   >  1:45242/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACATTATGCTGGTTGTCGACCACTc   >  1:2192/1‑60 (MQ=255) tCGCACCAAACGCGCCATCATTGAGAACTCGCGCCACATTATGCTGGTTGTCGACCACTc   >  1:2100/1‑60 (MQ=255)                                                       |       TCGCACCAAACGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCG  >  W3110S.gb/4080378‑4080438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑