Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,111,885	C→T	E689K (GAG→AAG)	yrfF ←	predicted inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,111,885	0	C	T	100.0%	43.9 / NA	26	E689K (GAG→AAG)	yrfF	predicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/0);  total (26/0)

TGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCT  >  W3110S.gb/4111848‑4111908                                      |                        tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGgcgc   >  1:86311/1‑60 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:53052/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:84283/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:83278/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:80041/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:67317/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:66401/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:6337/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:6234/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:60959/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:58251/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:56044/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:54524/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:11087/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:51726/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:45538/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:40408/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:38049/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:37599/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:37048/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:33543/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:32646/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:31352/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:29591/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:21298/1‑61 (MQ=255) tGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTTCATCATGCGAGTACGATGGCGCt  >  1:20710/1‑61 (MQ=255)                                      |                        TGCGGGTTCAGGCAGCTTTCATAATAGGCCTGAATCTCCATCATGCGAGTGCGATGACGCT  >  W3110S.gb/4111848‑4111908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑