Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,128,684	G→A	G204D (GGT→GAT)	yhfZ →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,128,684	0	G	A	100.0%	90.4 / NA	50	G204D (GGT→GAT)	yhfZ	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/50);  total (0/50)

TCGGCGGATCAGAAAATCATGACCGATGTTTTTTTTGGCGGTAGTGATGTGGAACGAGTCGAT  >  W3110S.gb/4128644‑4128706                                         |                       tCGGCAGATCAGAAAATCATGA‑CGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:48847/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGGACGAGTCGAt  <  1:87878/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:56229/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:55945/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:56351/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:59166/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:63086/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:6351/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:6460/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:64915/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:66671/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:67775/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:68617/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:68716/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:69351/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:72206/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:72294/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:73979/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:75105/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:77402/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:77722/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:78633/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:80166/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:80301/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:85567/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:87431/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:54854/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:12621/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:15101/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:15838/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:15851/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:19908/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:21942/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:2272/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:24576/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:25650/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:27066/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:32694/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:34203/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:39329/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:40199/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:40416/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:44066/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:50939/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:51248/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:53366/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:10800/62‑1 (MQ=255)  cggcagATCAGAAAATCATGACCGATGTCGTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:10922/62‑1 (MQ=255)    gcagATCAGAAAATCATGACCGATGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:18033/57‑1 (MQ=255)                          aTGTCTTTTTTGGCGATAGTGATGTGGAACGAGTCGAt  <  1:4816/38‑1 (MQ=38)                                         |                       TCGGCGGATCAGAAAATCATGACCGATGTTTTTTTTGGCGGTAGTGATGTGGAACGAGTCGAT  >  W3110S.gb/4128644‑4128706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑