Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,153,950	A→G	L117L (TTG→CTG)	crp ←	DNA‑binding transcriptional dual regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,153,950	0	A	G	100.0%	57.8 / NA	33	L117L (TTG→CTG)	crp	DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/0);  total (33/0)

GCAGACAAACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGA  >  W3110S.gb/4153943‑4154004        |                                                       gCAGACAGACGCTTCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:45715/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAAtt            >  1:64536/1‑52 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACg   >  1:19483/1‑61 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:8273/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:82177/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:74290/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:67442/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:65600/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:42605/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:6268/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:60652/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:6058/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:58642/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:54728/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:52880/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:48055/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:83893/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:43109/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:10041/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:37427/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:34447/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:32799/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:31514/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:2922/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:25735/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:24155/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:20591/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:20228/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:18394/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:17812/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:12855/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGa  >  1:12095/1‑62 (MQ=255) gCAGACAGACGCATCAGAATGTCAGGGTTTACCTGAATCAATTGGTGAAATTTTTTGTACGa  >  1:20842/1‑62 (MQ=255)        |                                                       GCAGACAAACGCATCAGAATGTCCGGGTTTACCTGAATCAATTGGCGAAATTTTTTGTACGA  >  W3110S.gb/4153943‑4154004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑