Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,248,178	C→T	A164T (GCC→ACG)	malF ←	maltose transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,248,178	0	C	T	100.0%	65.2 / NA	39	A164T (GCC→ACG)	malF	maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/39);  total (0/39)

CGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAA  >  W3110S.gb/4248155‑4248217                        |                                        cgcgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATT‑CAGTTTTTGCTCGCCGCCaaa  <  1:42089/62‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:63965/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:49304/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:56452/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:56849/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:60624/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:6119/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:6132/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:61332/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:62071/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:44876/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:66167/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:67795/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:69728/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:71941/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:74073/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:74749/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:75731/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:81697/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:82675/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:28501/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:12601/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:14687/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:17421/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:17632/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:21401/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:22060/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:26316/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:27323/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:27969/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:48026/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:32096/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:33229/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:35515/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:38745/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:39120/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:42155/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:42337/61‑1 (MQ=255)   cgcgCTCGCCTTCAGGCTGCGTGGCGCTTTCTTTCAATTGCAGTTTTTGCTCGCCGCCaaa  <  1:10/61‑1 (MQ=255)                        |                                        CGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAA  >  W3110S.gb/4248155‑4248217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑