Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,293,708 C→T V103V (GTC→GTT)  nrfC → formate‑dependent nitrite reductase, 4Fe4S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,293,7080CT100.0% 144.8 / NA 80V103V (GTC→GTT) nrfCformate‑dependent nitrite reductase, 4Fe4S subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/80);  total (0/80)

CAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGA  >  W3110S.gb/4293676‑4293734
                                |                          
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:59576/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:69468/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:69248/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:68138/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:67008/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:65413/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:63754/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:63091/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:60916/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:60380/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:71439/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:59167/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:58237/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:58186/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:57991/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:57968/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:57851/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:57012/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:53695/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:75892/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:8987/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:8914/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:88486/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:86284/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:83314/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:82118/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:8137/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:80199/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:7930/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:11529/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:74705/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:7438/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:73654/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:73590/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:72994/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:72830/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:72223/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:71495/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:18178/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:28787/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:284/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:53641/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:25663/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:24939/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:21434/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:20270/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:20078/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:18568/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:26961/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:15231/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:15137/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:14677/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:14473/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:1421/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:1283/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:12726/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:11109/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:36055/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:5333/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:51189/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:50717/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:47033/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:40998/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:38429/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:38194/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:29516/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:36032/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:35876/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:34690/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:32832/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:31652/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:30813/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:3078/59‑1 (MQ=255)
cAGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGGCTTTTCGCGa  <  1:15730/59‑1 (MQ=37)
cAGCACTGCGATCATGCGCCGGCCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:55417/59‑1 (MQ=37)
 aGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:25786/58‑1 (MQ=255)
 aGCACTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:66075/58‑1 (MQ=255)
     cTGCGATCATGCGCCGTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:2097/54‑1 (MQ=255)
                    gTGCGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:78004/39‑1 (MQ=255)
                       cGTTGACGTTTGCCCGACCGGTGCGTCTTTTCGCGa  <  1:79550/36‑1 (MQ=255)
                                |                          
CAGCACTGCGATCATGCGCCGTGCGTTGACGTCTGCCCGACCGGTGCGTCTTTTCGCGA  >  W3110S.gb/4293676‑4293734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑