Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,327,456	G→A	pseudogene (36/369 nt) pseudogene (506/621 nt)	phnE ← phnE ←	ECK4096:JW4064:b4103; phosphonate/organophosphate ester transporter phosphonate/organophosphate ester transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,327,456	0	G	A	100.0%	125.5 / NA	70	pseudogene (36/369 nt) pseudogene (506/621 nt)	phnE phnE	ECK4096:JW4064:b4103; phosphonate/organophosphate ester transporter phosphonate/organophosphate ester transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (70/0);  total (70/0)

TTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCAC  >  W3110S.gb/4327438‑4327499                   |                                            ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAgcgc                >  1:48444/1‑48 (MQ=25) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:59656/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:69268/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:68056/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:68017/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:66742/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:65841/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:63611/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:61192/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:59933/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:69973/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:58964/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:58542/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:57908/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:55983/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:55740/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:55280/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:54606/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:79030/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:85927/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:84716/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:83961/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:83543/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:83093/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:82768/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:8196/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:80680/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:11222/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:78714/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:78621/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:77471/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:77045/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:76723/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:73952/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:73810/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:21509/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:34213/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:33807/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:329/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:32536/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:28788/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:26343/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:25612/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:24942/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:52287/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:19694/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:18596/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:17643/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:17531/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:15462/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:14751/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:10675/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:3467/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:52435/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:49160/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:48490/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:48223/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:47582/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:47563/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:47329/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:46177/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:45517/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:44650/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:42639/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:41342/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:39069/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:36636/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcga  >  1:34466/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACCGGTGGTGTGGATAAACAGcgccagcacgccagcg   >  1:82193/1‑60 (MQ=255) ttCGGAAAGCAGTTTGGAAAGTACACAGGTGGTGGGGATAAACAGcgccagcacgccagcga  >  1:86707/1‑60 (MQ=255)                   |                                            TTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCAC  >  W3110S.gb/4327438‑4327499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑