Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,333,362	T→G	I516M (ATT→ATG)	yjdA →	conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,333,362	0	T	G	100.0%	114.2 / NA	62	I516M (ATT→ATG)	yjdA	conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (62/0);  total (62/0)

GATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAA  >  W3110S.gb/4333339‑4333400                        |                                       gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGGGCAGaa  >  1:35184/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:68756/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:57287/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:57307/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:57444/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:59570/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:5961/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:61014/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:61300/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:62469/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:62930/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:64730/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:65071/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:65192/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:6619/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:67865/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:56531/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:70560/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:70819/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:70825/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:7226/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:72315/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:73241/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:75400/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:7722/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:79101/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:80419/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:84247/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:87135/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:87614/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:88216/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:9521/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:25212/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:10103/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:119/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:19293/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:19852/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:20013/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:20433/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:21299/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:21354/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:21818/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:21825/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:22851/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:23194/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:24490/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:10054/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:31621/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:37238/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:37579/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:37771/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:42854/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:45747/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:46451/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:47151/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:48217/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:50179/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:50602/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:51081/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:51799/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACGGCGCAGaa  >  1:54688/4‑62 (MQ=255) gactCGGAGTCGTTAAGTGAGATGCGTACCTGCTGTGAGACACTGTTACAGACCGCGCAGaa  >  1:17899/4‑62 (MQ=255)                        |                                       GATTCGGAGCCGTTAAGCGAGATTCGTACCCGCTGTGAGACACTGTTACAGACGGCGCAGAA  >  W3110S.gb/4333339‑4333400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑