Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,340,665	C→T	E349E (GAG→GAA)	adiC ←	arginine:agmatin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,340,665	0	C	T	100.0%	34.9 / NA	22	E349E (GAG→GAA)	adiC	arginine:agmatin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/22);  total (0/22)

AGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCT  >  W3110S.gb/4340643‑4340699                       |                                   acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCTAAATGCTACTGAGCt  <  1:88548/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:66298/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:88475/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:88449/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:88352/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:84344/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:81740/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:77818/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:72465/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:7147/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:10125/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:58823/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:54985/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:39771/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:37378/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:36322/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:30678/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:20894/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:17388/55‑1 (MQ=255) acaCGGAAGAAACCAGACCGAATTCTTTAGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:39427/55‑1 (MQ=255)  caCGGAAGAAACCAGACCGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:73651/55‑1 (MQ=255)                   cGAATTCTTTGGTCGCGTTTGGCGAAATGCTACTGAGCt  <  1:52071/39‑1 (MQ=25)                       |                                   AGACGGAAGAAACCAGACCGAACTCTTTGGTCGCGTTTGGTGAAATGCTGCTGAGCT  >  W3110S.gb/4340643‑4340699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑