Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,370,009 C→T A10T (GCG→ACG)  cutA ← copper binding protein, copper sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,370,0090CT98.9% 174.1 / NA 94A10T (GCG→ACG) cutAcopper binding protein, copper sensitivity
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (93/0);  minor base A (1/0);  total (94/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACC  >  W3110S.gb/4369982‑4370043
                           |                                  
gagCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:64243/3‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:31128/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:31620/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:42169/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:73035/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:19228/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:29222/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:20141/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:40784/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAaca    >  1:67358/1‑60 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:60913/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:65679/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:6541/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:65096/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:65014/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:64074/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:63557/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:62427/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:71851/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:57693/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:56179/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:55709/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:54909/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:53412/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:51610/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:48878/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:4887/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:79463/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:9946/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:9187/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:87855/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:86515/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:83368/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:83186/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:82076/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:80732/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:80569/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:79470/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:68850/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:79078/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:79000/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:76629/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:76254/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:74428/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:74332/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:74002/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:73172/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:44674/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:70488/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:22805/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:48297/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:30603/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:30599/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:29316/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:29011/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:2871/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:28265/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:27778/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:24924/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:23580/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:30808/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:21890/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:2148/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:21414/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:19548/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:18823/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:17312/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:16871/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:16171/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:15911/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:14257/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:39187/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:44872/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:12068/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:42312/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:42077/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:41574/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:410/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:40403/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:39357/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:39025/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:38088/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:37340/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:36021/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:33642/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:32897/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:32122/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:31946/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:31875/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTACATCAAGCATAAACAcc  >  1:57649/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGTGGTATTCCAACTTTTTTCATCAAGCATAAACAcc  >  1:57493/1‑62 (MQ=255)
gTGCCGTACATAGCACCACGACAGACGAGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:30822/1‑61 (MQ=255)
gTGCCGTACATAGCACCACGAAAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAACAcc  >  1:25044/1‑62 (MQ=255)
gTGACGTACATAGCACCACGACAGACGTGGTATTCGAACTTTTTTCATCAAGCATAAacac   >  1:9756/1‑61 (MQ=255)
                           |                                  
GTGCCGTACATAGCACCACGACAGACGCGGTATTCGAACTTTTTTCATCAAGCATAAACACC  >  W3110S.gb/4369982‑4370043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑