Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,373,316 G→T intergenic (‑307/‑30) aspA ← / → fxsA aspartate ammonia‑lyase/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,373,3160GT100.0% 13.7 / NA 11intergenic (‑307/‑30)aspA/fxsAaspartate ammonia‑lyase/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/11);  total (0/11)

AATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCGGCTGCTATTAC  >  W3110S.gb/4373273‑4373334
                                           |                  
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:16864/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:24431/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:25190/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:37608/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:41991/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:45024/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:51552/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:63631/62‑1 (MQ=255)
aaTATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:67648/62‑1 (MQ=255)
 atatAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:12568/61‑1 (MQ=255)
 atatAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGTTTAACCGGCTGCTATTAc  <  1:13704/61‑1 (MQ=255)
                                           |                  
AATATAAGCTAAACGCGATTCGCAACCCATTCAGGTAGCCGGGGTTAACCGGCTGCTATTAC  >  W3110S.gb/4373273‑4373334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑