Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,402,255 C→T I54I (ATC→ATT)  mutL → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,402,2550CT100.0% 186.8 / NA 102I54I (ATC→ATT) mutLmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/102);  total (0/102)

Only 100 of 102 total aligned reads displayed.

CGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTG  >  W3110S.gb/4402214‑4402275
                                         |                    
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAATTTATTCGCATTCGTGATAACGGCTg  <  1:74877/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:57520/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:6610/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:64762/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:63995/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:61936/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:60136/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:59883/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:58838/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:58002/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:57751/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:57726/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:57551/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:67563/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:57032/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:56291/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:56147/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:53652/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:52595/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:5245/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:51852/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:51619/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:50858/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:10860/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:49112/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:79608/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:9106/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:9088/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:88261/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:88170/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:87507/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:8555/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:85546/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:85319/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:84431/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:81354/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:80351/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:66939/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:79269/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:78274/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:756/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:73720/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:73579/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:73356/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:72262/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:7182/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:70096/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:68836/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:50672/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:18534/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:24485/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:48174/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:22402/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:22317/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:22204/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:21903/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:20658/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:20593/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:20161/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:1980/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:1955/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:23876/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:16410/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:16192/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:15416/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:13886/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:13714/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:13043/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:12804/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:12091/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:11909/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:11266/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:25234/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:47645/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:44956/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:44773/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:44370/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:43293/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:4165/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:40897/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:40503/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:39293/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:36751/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:35913/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:3405/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:32473/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:32286/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:32232/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:29776/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:29206/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:28346/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:27737/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:26008/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:25248/62‑1 (MQ=255)
cgTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:24905/62‑1 (MQ=255)
 ttATCGATATTGATATCGAACGCGGTGGTGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:74750/60‑1 (MQ=255)
 gTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:74836/61‑1 (MQ=255)
          ttGATATCGAACGCGGTGGGGCGAAACTTATTCGCATTCGTGATAACGGCTg  <  1:84480/52‑1 (MQ=255)
                        ggTGGGGCGAAACTTATTAGCATTCGTGATAACGGCTg  <  1:1273/38‑1 (MQ=38)
                          tGGGGCGAAACTTATTCGCTTTCGTGATAACGGCTg  <  1:18740/36‑1 (MQ=37)
                                         |                    
CGTATCGATATTGATATCGAACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTG  >  W3110S.gb/4402214‑4402275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑