Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,420,631	G→T	intergenic (+49/+68)	aidB → / ← yjfN	isovaleryl CoA dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,420,631	0	G	T	100.0%	46.2 / NA	28	intergenic (+49/+68)	aidB/yjfN	isovaleryl CoA dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/0);  total (28/0)

ATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGC  >  W3110S.gb/4420627‑4420686     |                                                        atatTTTAAAATTGTCGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:8599/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:55425/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:87478/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:87382/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:85936/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:82331/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:78963/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:77002/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:7579/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:70721/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:66495/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:61651/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:61045/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:59194/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:10154/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:53511/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:51220/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:51127/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:50972/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:48276/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:40791/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:36300/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:33176/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:2861/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:25879/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:17265/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGc  >  1:13921/1‑60 (MQ=21) atatTTTAAAATTGTAGGCCGGACAAGGCGCACGCGCCGCATCCGGCATTGTTCATTTGc  >  1:44447/1‑60 (MQ=21)     |                                                        ATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGC  >  W3110S.gb/4420627‑4420686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑