Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,439,838 G→A P470P (CCC→CCT)  cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,439,8380GA100.0% 138.3 / NA 78P470P (CCC→CCT) cpdB2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (78/0);  total (78/0)

TAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTG  >  W3110S.gb/4439788‑4439849
                                                  |           
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:59860/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:69954/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:69111/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:67274/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:65497/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:64589/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:64107/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:62174/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:61338/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:60487/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:70739/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:56906/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:56791/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:56227/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:56090/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:54932/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:53869/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:5267/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:49245/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:10015/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:84204/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:9451/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:8997/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:8996/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:87049/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:86699/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:85866/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:85547/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:85453/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:84786/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:48435/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:82702/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:82257/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:81730/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:80402/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:79547/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:75139/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:73416/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:72079/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:17841/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:27629/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:26790/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:26778/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:25841/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:25798/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:2577/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:24998/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:19016/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:18595/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:27723/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:17757/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:16667/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:14876/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:14634/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:142/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:1393/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:13867/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:11402/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:10672/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:40610/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:47982/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:47179/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:46412/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:45380/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:45272/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:4375/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:43372/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:42272/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:39631/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:37267/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:36805/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:32879/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:32668/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:32303/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:28673/4‑62 (MQ=255)
taggTGCGGAAACCATCCCAGGTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:42792/4‑62 (MQ=255)
taggTGCGGAAA‑CATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:66594/4‑61 (MQ=255)
taggTGCGCAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTAGGATCAATCTg  >  1:48155/4‑62 (MQ=255)
                                                  |           
TAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTG  >  W3110S.gb/4439788‑4439849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑