Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,458,330	C→T	C22C (TGC→TGT)	yjfF →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,458,330	0	C	T	100.0%	81.2 / NA	45	C22C (TGC→TGT)	yjfF	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/0);  total (45/0)

CTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATA  >  W3110S.gb/4458327‑4458388    |                                                           cTGTCTGCCCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:62962/1‑62 (MQ=255) cTGTCTGACCCAGTTTGCCGGTTTTGCTTCCAGAAGAGTGAt                      >  1:31593/1‑42 (MQ=37) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAatat             >  1:39452/1‑51 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGaaa  >  1:78402/1‑60 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGAt   >  1:977/1‑61 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGAt   >  1:3323/1‑61 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGAt   >  1:27081/1‑61 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:88568/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:51150/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:52309/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:53313/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:56272/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:58466/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:58795/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:62018/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:62809/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:47838/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:64191/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:66999/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:85077/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:73217/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:75775/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:81762/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:77306/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:80943/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:35527/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:14623/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:1811/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:18488/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:19669/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:2537/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:30370/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:30915/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:31769/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:31771/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:50926/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:37882/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:39523/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:40861/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:42758/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:44210/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:44460/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:45266/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:13185/1‑62 (MQ=255) cTGTCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATa  >  1:4926/1‑62 (MQ=255)    |                                                           CTGCCTGACCCAGTTTCCCGGTTTTGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATA  >  W3110S.gb/4458327‑4458388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑